O-linked N-acetylglucosamine transferase (OGT) regulates pancreatic α-cell function in mice.

ABSTRACT: The nutrient sensor O-GlcNAc transferase (OGT) catalyzes posttranslational addition of O-GlcNAc onto target proteins, influencing signaling pathways in response to cellular nutrient levels. OGT is highly expressed in pancreatic glucagon-secreting cells (α-cells), which secrete glucagon in response to hypoglycemia. The objective of this study was to determine whether OGT is necessary for the regulation of α-cell mass and function in vivo. We utilized genetic manipulation to produce two α-cell specific OGT-knockout models: a constitutive glucagon-Cre (αOGT^KO) and an inducible glucagon-Cre (i-αOGT^KO), which effectively delete OGT in α-cells. Using approaches including immunoblotting, immunofluorescent imaging, and metabolic phenotyping in vivo, we provide the first insig

SUBMITTER: Essawy A 

PROVIDER: S-EPMC7949098 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature