Mapping membrane lipids in the developing and adult mouse retina under physiological and pathological conditions using mass spectrometry.

ABSTRACT: Membrane phospholipids play pivotal roles in various cellular processes, and their levels are tightly regulated. In the retina, phospholipids had been scrutinized because of their distinct composition and requirement in visual transduction. However, how lipid composition changes during retinal development remains unclear. Here, we used liquid chromatography-mass spectrometry (LC-MS) to assess the dynamic changes in the levels of two main glycerophospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), in the developing mouse retina under physiological and pathological conditions. The total levels of PC and PE increased during retinal development, and individual lipid species exhibited distinct level changes. The amount of very-long-chain PC and PE increased dramatically in

SUBMITTER: Hamano F 

PROVIDER: S-EPMC7949107 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature