Trans-2-enoyl-CoA reductase limits Ca²⁺ accumulation in the endoplasmic reticulum by inhibiting the Ca²⁺ pump SERCA2b.

ABSTRACT: The endoplasmic reticulum (ER) contains various enzymes that metabolize fatty acids (FAs). Given that FAs are the components of membranes, FA metabolic enzymes might be associated with regulation of ER membrane functions. However, it remains unclear whether there is the interplay between FA metabolic enzymes and ER membrane proteins. Trans-2-enoyl-CoA reductase (TER) is an FA reductase present in the ER membrane and catalyzes the last step in the FA elongation cycle and sphingosine degradation pathway. Here we identify sarco(endo)plasmic reticulum Ca²⁺-ATPase 2b (SERCA2b), an ER Ca²⁺ pump responsible for Ca²⁺ accumulation in the ER, as a TER-binding protein by affinity purification from HEK293 cell lysates. We show that TER directly binds to SERCA2b by in v

SUBMITTER: Uchida Y 

PROVIDER: S-EPMC7949109 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature