Angiotensin II increases activity of the ClC-K2 Cl^- channel in collecting duct intercalated cells by stimulating production of reactive oxygen species.

ABSTRACT: The renal collecting duct plays a critical role in setting urinary volume and composition, with principal cells transporting Na⁺ and K⁺ and intercalated cells mediating Cl^- reabsorption. Published evidence implies Angiotensin II (Ang II) is a potent regulator of the collecting duct apical transport systems in response to systemic volume depletion. However, virtually nothing is known about Ang II actions on the basolateral conductance of principal and intercalated cells. Here, we combined macroscopic and single channel patch clamp recordings from freshly isolated mouse collecting ducts with biochemical and fluorescence methods to demonstrate an acute stimulation of the basolateral Cl^- conductance and specifically the ClC-K2 Cl^- channel

SUBMITTER: Khayyat NH 

PROVIDER: S-EPMC7949157 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature