Promotion of acute-phase skin wound healing by Pseudomonas aeruginosa C₄ -HSL.

ABSTRACT: A Pseudomonas aeruginosa quorum-sensing system, which produces N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C₁₂ -HSL) and N-butanoyl-l-homoserine lactone (C₄ -HSL), regulates the virulence factors. In our previous study, 3-oxo-C₁₂ -HSL, encoded by lasI gene, was shown to promote wound healing. However, the effect of C₄ -HSL, encoded by rhlI gene, remains to be elucidated. We addressed the effect of C₄ -HSL on wounds in P. aeruginosa infection. Wounds were created on the backs of Sprague-Dawley SD rats, and P. aeruginosa PAO1 (PAO1) or its rhlI deletion mutant (ΔrhlI) or lasI deletion mutant (ΔlasI) was inoculated onto the wound. Rats were injected intraperitoneally with anti-C₄ -HSL antiserum or treated with C₄ -HSL at the wound surface. PAO1 inoculation led to significant acceleration of wound healing, which was associated with neutrophil infiltration and TNF-α synthesis. These responses were reversed, except for TNF-α production, when ΔrhlI was inoculated instead of PAO1 or when rats were co-treated with PAO1 and anti-C₄ -HSL antiserum. In contrast, the healing process and neutrophil infiltration, but not TNF-α synthesis, were accelerated when C₄ -HSL was administered in the absence of PAO1. This acceleration was not affected by anti-TNF-α antibody. These results suggest that C₄ -HSL may be involved in the acceleration of acute wound healing in P. aeruginosa infection by modifying the neutrophilic inflammation.

SUBMITTER: Kanno E 

PROVIDER: S-EPMC7949945 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature