An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age.

ABSTRACT: The process of aging has been associated with differential patterns of DNA methylation which relate to changes in gene expression. Hence, we aimed to identify genes with significant age-related methylation differences and to study their mRNA and protein expression profile. Genome-wide DNA methylation analysis was performed with the Illumina Infinium Methylation EPIC BeadChip Microarray on bisulfite-converted DNA prepared from monocytes derived from young (average age: 23.8 yrs) and old (average age: 81.5 yrs) volunteers that are separated by at least 50 years of age difference, n=4, respectively. Differentially methylated CpG sites were assigned to the associated genes and validated by deep sequencing analysis (n=20). Demonstrating an age-associated significant increase of methylation in t

SUBMITTER: Feiner LK 

PROVIDER: S-EPMC7950283 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature