Insights into epithelial cell senescence from transcriptome and secretome analysis of human oral keratinocytes.

ABSTRACT: Senescent cells produce chronic inflammation that contributes to the diseases and debilities of aging. How this process is orchestrated in epithelial cells, the origin of human carcinomas, is poorly understood. We used human normal oral keratinocytes (NOKs) to elucidate senescence programs in a prototype primary mucosal epithelial cell that senesces spontaneously. While NOKs exhibit several typical facets of senescence, they also display distinct characteristics. These include expression of p21WAF1/CIP1 at early passages, making this common marker of senescence unreliable in NOKs. Transcriptome analysis by RNA-seq revealed specific commonalities with and differences from cancer cells, explicating the tumor avoidance role of senescence. Repression of DNA repair genes that correlated with do

SUBMITTER: Schwartz RE 

PROVIDER: S-EPMC7950289 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature