Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa.

ABSTRACT: Quinoa depicts high nutritional quality and abiotic stress resistance, attracting strong interest in the last years. To unravel the function of candidate genes for agronomically relevant traits, studying their transcriptional activities by RT-qPCR is an important experimental approach. The accuracy of such experiments strongly depends on precise data normalization. To date, validation of potential candidate genes for normalization of diurnal expression studies has not been performed in C. quinoa. We selected eight candidate genes based on transcriptome data and literature survey, including conventionally used reference genes. We used three statistical algorithms (BestKeeper, geNorm and NormFinder) to test their stability and added further validation by a simulation-based strategy. We demon

SUBMITTER: Maldonado-Taipe N 

PROVIDER: S-EPMC7951847 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature