Interspecies chimeric conditions affect the developmental rate of human pluripotent stem cells.

ABSTRACT: Human pluripotent stem cells hold significant promise for regenerative medicine. However, long differentiation protocols and immature characteristics of stem cell-derived cell types remain challenges to the development of many therapeutic applications. In contrast to the slow differentiation of human stem cells in vitro that mirrors a nine-month gestation period, mouse stem cells develop according to a much faster three-week gestation timeline. Here, we tested if co-differentiation with mouse pluripotent stem cells could accelerate the differentiation speed of human embryonic stem cells. Following a six-week RNA-sequencing time course of neural differentiation, we identified 929 human genes that were upregulated earlier and 535 genes that exhibited earlier peaked expression profiles in chi

SUBMITTER: Brown J 

PROVIDER: S-EPMC7951976 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature