Long-Term Stability of 18 Nutritional Biomarkers Stored at -20 °C and 5 °C for up to 12 Months.

ABSTRACT:

Background

Consistent information on long-term storage stability for a broad range of nutritional biomarkers is lacking. We investigated the stability of 18 biomarkers stored at suboptimal temperatures (-20 °C and 5 °C) for up to 12 months.

Methods

Multiple vials of serum or whole blood pools (3 concentrations) were stored at -20 °C or 5 °C, removed from the -20 °C freezer after 3, 6, 9, and 12 months and from the 5 °C refrigerator after 6 and 12 months, and placed into a -70 °C freezer until analysis at study completion. Vials stored continuously at -70 °C were used as the reference condition for optimal storage. We measured 18 biomarkers: 4 iron status, 1 inflammation, 8 water-soluble vitamin, and 5 fat-soluble vitamin. For each temperature, we calculated geometric mean co

SUBMITTER: Chen H 

PROVIDER: S-EPMC7951994 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature