Unactivated leukocyte expression of C-reactive protein is minimal and not dependent on rs1205 genotype.

ABSTRACT: C-reactive protein (CRP), a prominent component of the innate immune system, is implicated in the pathophysiology of many conditions. CRP production primarily occurs in the liver; but contributions from other tissues is unclear. The Genotype-Tissue Expression Portal shows essentially no expression in whole blood and reports in the literature are conflicting. Multiple genomic variants influence serum levels of CRP. We measured CRP mRNA expression in leukocytes and sought to determine if rs1205 genotype influences leukocyte expression. Leukocytes were obtained from 20 women differing by genotype. Quantitative, real-time PCR (RT-qPCR) detected CRP and reference gene (GAPDH) mRNA. Leukocyte expression was calculated by the 2^ΔCT method, and against a standard curve. Digital drop PCR

SUBMITTER: Best LG 

PROVIDER: S-EPMC7952394 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature