Comparison of MiSeq, MinION, and hybrid genome sequencing for analysis of Campylobacter jejuni.

ABSTRACT: The sequencing, assembly, and analysis of bacterial genomes is central to tracking and characterizing foodborne pathogens. The bulk of bacterial genome sequencing at the US Food and Drug Administration is performed using short-read Illumina MiSeq technology, resulting in highly accurate but fragmented genomic sequences. The MinION sequencer from Oxford Nanopore is an evolving technology that produces long-read sequencing data with low equipment cost. The goal of this study was to compare Campylobacter genome assemblies generated from MiSeq and MinION data independently, as well as hybrid genome assemblies combining both data types. Two reference strains and two field isolates of C. jejuni were sequenced using MiSeq and MinION, and the sequence data were assembled using the software program

SUBMITTER: Neal-McKinney JM 

PROVIDER: S-EPMC7952698 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature