Tumor-educated leukocytes mRNA as a diagnostic biomarker for non-small cell lung cancer.

ABSTRACT:

Background

This study aimed to investigate the diagnostic and prognostic role of tumor-educated leukocytes (TELs) mRNA in Chinese patients with non-small cell lung cancer (NSCLC).

Methods

The TELs collected underwent total RNA isolation. RNA-sequencing (RNA-seq) technology was used to analyze the transcriptome of the TELs. The mRNA expression levels of differential genes were analyzed by RT-qPCR. Statistical analyses were performed using Prism and SPSS by Mann-Whitney nonparametric test, Kruskal-Wallis test and one-way ANOVA.

Results

We used RNA-seq technology to screen 95 differential genes (DEGs) from seven NSCLC and four controls, wherein 15 genes were upregulated, and 80 were downregulated. Of these, four genes were selected for further analysis, wherein one was u

SUBMITTER: Niu L 

PROVIDER: S-EPMC7952788 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature