Global multi-method analysis of interaction parameters for reversibly self-associating macromolecules at high concentrations.

ABSTRACT: Weak macromolecular interactions assume a dominant role in the behavior of highly concentrated solutions, and are at the center of a variety of fields ranging from colloidal chemistry to cell biology, neurodegenerative diseases, and manufacturing of protein drugs. They are frequently measured in different biophysical techniques in the form of second virial coefficients, and nonideality coefficients of sedimentation and diffusion, which may be related mechanistically to macromolecular distance distributions in solution and interparticle potentials. A problem arises for proteins where reversible self-association often complicates the concentration-dependent behavior, such that grossly inconsistent coefficients are measured in experiments based on different techniques, confounding quantitativ

SUBMITTER: Parupudi A 

PROVIDER: S-EPMC7952917 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature