Non-radioactive Assay to Determine Product Profile of Short-chain Isoprenyl Diphosphate Synthases.

ABSTRACT: Isoprenoids represent the largest class of metabolites with amazing diversities in structure and function. They are involved in protecting plants against pathogens or herbivores or involved in attracting pollinators. Isoprenoids are derived from geranyl diphosphate (GPP; C₁₀), farnesyl diphosphate (FPP; C₁₅), geranylgeranyl diphosphate (GGPP; C₂₀), and geranylfarnesyl diphosphate (GFPP; C₂₅) that are in turn formed by sequential condensations of isopentenyl diphosphate (IPP; C₅) with an allylic acceptor such as dimethylallyl diphosphate (DMAPP; C₅), GPP, FPP, or GGPP in a reaction catalyzed by isoprenyl diphosphate synthases (IDSs). IDS enzyme assay for determination of prenyl diphosphate products is generally performed usin

SUBMITTER: Rai A 

PROVIDER: S-EPMC7952919 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature