Measuring Extracellular Proton and Anionic Fluxes in Arabidopsis Pollen Tubes.

ABSTRACT: The ion-selective vibrating probe has been used to detect and quantify the magnitude and direction of transmembrane fluxes of several ions in a wide range of biological systems. Inherently non-invasive, vibrating probes have been essential to access relevant electrophysiological parameters related to apical growth and morphogenesis in pollen tubes, a highly specialized cell where spatiotemporal tuning of ion dynamics is fundamental. Of relevance, crucial processes to the cell physiology of pollen tubes associated with protons and anions have been elucidated using vibrating probes, allowing the identification of diverse molecular players underlying and regulating their extracellular fluxes. The use of Arabidopsis thaliana as a genetic model system posed new challenges given their rel

SUBMITTER: Portes MT 

PROVIDER: S-EPMC7952931 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature