Method comparison of targeted influenza A virus typing and whole-genome sequencing from respiratory specimens of companion animals.

ABSTRACT: Epidemics of H3N8 and H3N2 influenza A viruses (IAVs) in dogs, along with recognition of spillover infections from IAV strains typically found in humans or other animals, have emphasized the importance of efficient laboratory testing. Given the lack of active IAV surveillance or immunization requirements for dogs, cats, or horses imported into the United States, serotype prediction and whole-genome sequencing of positive specimens detected at veterinary diagnostic laboratories are also needed. The conserved sequences at the ends of the viral genome segments facilitate universal amplification of all segments of viral genomes directly from respiratory specimens. Although several methods for genomic analysis have been reported, no optimization focusing on companion animal strains has been des

SUBMITTER: Mitchell PK 

PROVIDER: S-EPMC7953085 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature