Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction.

ABSTRACT:

Background

Compare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin.

Methodology

Dentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S¹ binding-domain with k21.

Results

There was reduction of E.

SUBMITTER: Kok ESK 

PROVIDER: S-EPMC7953794 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature