Lanthanide-Doped Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Carbohydrate Antigen 19-9.

ABSTRACT: Lanthanide-doped upconversion nanoparticles (UCNPs) have attracted considerable attention in detection of biological analytes and bioimaging owing to their superior optical properties, including high photochemical stability, sharp emission bandwidth, large anti-Stokes shifts, and low toxicity. In this work, we fabricated UCNP-linked immunosorbent assay (ULISA) for the sensitive detection of carbohydrate antigen 19-9 (CA19-9). The design is based on amino-functionalized SiO₂-coated Gd-doped NaYF₄:Yb³⁺,Er³⁺ upconversion nanoparticles (UCNPs@SiO₂-NH₂) as a direct background-free luminescent reporter; a secondary anti-IgG antibody (Ab₂) was conjugated to the surface of UCNPs@SiO₂-NH₂ (UCNP-Ab

SUBMITTER: Zhou C 

PROVIDER: S-EPMC7954120 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature