A new monoclonal antibody that blocks dimerisation and inhibits c-kit mutation-driven tumour growth.

ABSTRACT:

Purpose

Imatinib, a small-molecule tyrosine kinase inhibitor, has shown good clinical activity by inhibiting adenosine triphosphate (ATP) binding to the receptor. Unfortunately, majority of patients eventually develop drug resistance, which limits the long-term benefits of the tyrosine kinase inhibitors and poses a significant challenge in the clinical management of GIST. The aim of our study was to explore the feasibility of blocking KIT dimerisation upstream of the phosphorylation in imatinib-resistant GIST.

Method

KITMAb was prepared using hybridoma technique. The biological function of KITMAb was examined in KIT-dimer-expressing cells constructed by transfecting with liposomes using enzyme linked immunosorbent assay (ELISA), immunohistochemistry, western blot, MTT, Annex

SUBMITTER: Bai C 

PROVIDER: S-EPMC7954730 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature