Protuberances are organized distinct regions of long-term callus: histological and transcriptomic analyses in kiwifruit.

ABSTRACT:

Key message

Macroscopic, ultrastructural, and molecular features-like a ball shape, the presence of starch granules, and the up-regulation of genes involved in carbohydrate metabolism and secondary metabolite biosynthesis-distinguish PT regions within a callus. The modification of the mass of pluripotent cells into de novo shoot bud regeneration is highly relevant to developmental biology and for agriculture and biotechnology. This study deals with protuberances (PT), structures that appear during the organogenic long-term culturing of callus (OC) in kiwifruit. These ball-shaped regions of callus might be considered the first morphological sign of the subsequent shoot bud development. Sections of PT show the regular arrangement of some cells, especially on the surface, in contrast

SUBMITTER: Czernicka M 

PROVIDER: S-EPMC7954764 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature