A computer-guided design tool to increase the efficiency of cellular conversions.

ABSTRACT: Human cell conversion technology has become an important tool for devising new cell transplantation therapies, generating disease models and testing gene therapies. However, while transcription factor over-expression-based methods have shown great promise in generating cell types in vitro, they often endure low conversion efficiency. In this context, great effort has been devoted to increasing the efficiency of current protocols and the development of computational approaches can be of great help in this endeavor. Here we introduce a computer-guided design tool that combines a computational framework for prioritizing more efficient combinations of instructive factors (IFs) of cellular conversions, called IRENE, with a transposon-based genomic integration system for efficient delivery. Part

SUBMITTER: Jung S 

PROVIDER: S-EPMC7954801 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature