Cell kinetics of auxin transport and activity in Arabidopsis root growth and skewing.

ABSTRACT: Auxin is a key regulator of plant growth and development. Local auxin biosynthesis and intercellular transport generates regional gradients in the root that are instructive for processes such as specification of developmental zones that maintain root growth and tropic responses. Here we present a toolbox to study auxin-mediated root development that features: (i) the ability to control auxin synthesis with high spatio-temporal resolution and (ii) single-cell nucleus tracking and morphokinetic analysis infrastructure. Integration of these two features enables cutting-edge analysis of root development at single-cell resolution based on morphokinetic parameters under normal growth conditions and during cell-type-specific induction of auxin biosynthesis. We show directional auxin flow in the r

SUBMITTER: Hu Y 

PROVIDER: S-EPMC7954861 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature