Cryo-EM structure of the Rous sarcoma virus octameric cleaved synaptic complex intasome.

ABSTRACT: Despite conserved catalytic integration mechanisms, retroviral intasomes composed of integrase (IN) and viral DNA possess diverse structures with variable numbers of IN subunits. To investigate intasome assembly mechanisms, we employed the Rous sarcoma virus (RSV) IN dimer that assembles a precursor tetrameric structure in transit to the mature octameric intasome. We determined the structure of RSV octameric intasome stabilized by a HIV-1 IN strand transfer inhibitor using single particle cryo-electron microscopy. The structure revealed significant flexibility of the two non-catalytic distal IN dimers along with previously unrecognized movement of the conserved intasome core, suggesting ordered conformational transitions between intermediates that may be important to capture the target DNA

SUBMITTER: Pandey KK 

PROVIDER: S-EPMC7955051 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature