The FlgN chaperone activates the Na⁺-driven engine of the Salmonella flagellar protein export apparatus.

ABSTRACT: The bacterial flagellar protein export machinery consists of a transmembrane export gate complex and a cytoplasmic ATPase complex. The gate complex has two intrinsic and distinct H⁺-driven and Na⁺-driven engines to drive the export of flagellar structural proteins. Salmonella wild-type cells preferentially use the H⁺-driven engine under a variety of environmental conditions. To address how the Na⁺-driven engine is activated, we analyzed the fliJ(Δ13-24) fliH(Δ96-97) mutant and found that the interaction of the FlgN chaperone with FlhA activates the Na⁺-driven engine when the ATPase complex becomes non-functional. A similar activation can be observed with either of two single-residue substitutions in FlhA. Thus, it is likely that the Fl

SUBMITTER: Minamino T 

PROVIDER: S-EPMC7955116 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature