Sympathetic Input to Multiple Cell Types in Mouse and Human Colon Produces Region-Specific Responses.

ABSTRACT:

Background & aims

The colon is innervated by intrinsic and extrinsic neurons that coordinate functions necessary for digestive health. Sympathetic input suppresses colon motility by acting on intrinsic myenteric neurons, but the extent of sympathetic-induced changes on large-scale network activity in myenteric circuits has not been determined. Compounding the complexity of sympathetic function, there is evidence that sympathetic transmitters can regulate activity in non-neuronal cells (such as enteric glia and innate immune cells).

Methods

We performed anatomical tracing, immunohistochemistry, optogenetic (GCaMP calcium imaging, channelrhodopsin), and colon motility studies in mice and single-cell RNA sequencing in human colon to investigate how sympathetic postganglionic ne

SUBMITTER: Smith-Edwards KM 

PROVIDER: S-EPMC7956113 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature