A Novel Role of SLC26A3 in the Maintenance of Intestinal Epithelial Barrier Integrity.

ABSTRACT:

Background & aims

The down-regulated in adenoma (DRA) protein, encoded by SLC26A3, a key intestinal chloride anion exchanger, has recently been identified as a novel susceptibility gene for inflammatory bowel disease (IBD). However, the mechanisms underlying the increased susceptibility to inflammation induced by the loss of DRA remain elusive. Compromised barrier is a key event in IBD pathogenesis. The current studies were undertaken to elucidate the impact of DRA deficiency on epithelial barrier integrity and to define underlying mechanisms.

Methods

Wild-type and DRA-knockout (KO) mice and crypt-derived colonoids were used as models for intestinal epithelial response. Paracellular permeability was measured by using fluorescein isothiocyanate-dextran flux. Immunoblotting, i

SUBMITTER: Kumar A 

PROVIDER: S-EPMC7956241 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature