The Angiogenic Potential of Mesenchymal Stem Cells from the Hair Follicle Outer Root Sheath.

ABSTRACT: Neovascularization is regarded as a pre-requisite in successful tissue grafting of both hard and soft tissues alike. This study considers mesenchymal stem cells from hair follicle outer root sheath (MSCORS) as powerful tools with a neat angiogenic potential that could in the future have wide scopes of neo-angiogenesis and tissue engineering. Autologous MSCORS were obtained ex vivo by non-invasive plucking of hair and they were differentiated in vitro into both endothelial cells and vascular smooth muscle cells (SMCs), two crucial cellular components of vascular grafts. Assessment was carried out by immunostaining, confocal laser-scanning microscopy, gene expression analysis (qRT-PCR), quantitative analysis of anastomotic network parameters, and cumulative length quantification of immunosta

SUBMITTER: Savkovic V 

PROVIDER: S-EPMC7956349 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature