Identification of m⁶A-Associated RNA Binding Proteins Using an Integrative Computational Framework.

ABSTRACT: N6-methyladenosine (m⁶A) is an abundant modification on mRNA that plays an important role in regulating essential RNA activities. Several wet lab studies have identified some RNA binding proteins (RBPs) that are related to m⁶A's regulation. The objective of this study was to identify potential m⁶A-associated RBPs using an integrative computational framework. The framework was composed of an enrichment analysis and a classification model. Utilizing RBPs' binding data, we analyzed reproducible m⁶A regions from independent studies using this framework. The enrichment analysis identified known m⁶A-associated RBPs including YTH domain-containing proteins; it also identified RBM3 as a potential m⁶A-associated RBP for mouse. Furthe

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7957075 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature