NGS-Based Application for Routine Non-Invasive Pre-Implantation Genetic Assessment in IVF.

ABSTRACT: Although non-invasive pre-implantation genetic testing for aneuploidy (NIPGT-A) is potentially appropriate to assess chromosomal ploidy of the embryo, practical application of it in a routine IVF centre have not been started in the absence of a recommendation. Our objective in this study was to provide a comprehensive workflow for a clinically applicable strategy for NIPGT-A based on next-generation sequencing (NGS) technology with the corresponding bioinformatic pipeline. In a retrospective study, we performed NGS on spent blastocyst culture media of Day 3 embryos fertilised with intracytoplasmic sperm injection (ICSI) with quality score on morphology assessment using the blank culture media as background control. Chromosomal abnormalities were identified by an optimised bioinformatics pi

SUBMITTER: Gombos K 

PROVIDER: S-EPMC7957524 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature