DNA Organization along Pachytene Chromosome Axes and Its Relationship with Crossover Frequencies.

ABSTRACT: During meiosis, the number of crossovers vary in correlation to the length of prophase chromosome axes at the synaptonemal complex stage. It has been proposed that the regular spacing of the DNA loops, along with the close relationship of the recombination complexes and the meiotic axes are at the basis of this covariation. Here, we use a cytogenomic approach to investigate the relationship between the synaptonemal complex length and the DNA content in chicken oocytes during the pachytene stage of the first meiotic prophase. The synaptonemal complex to DNA ratios of specific chromosomes and chromosome segments were compared against the recombination rates obtained by MLH1 focus mapping. The present results show variations in the DNA packing ratios of macro- and microbivalents and also betw

SUBMITTER: Del Priore L 

PROVIDER: S-EPMC7957551 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature