Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis.

ABSTRACT: From insects to mice, oocytes develop within cysts alongside nurse-like sister germ cells. Prior to fertilization, the nurse cells' cytoplasmic contents are transported into the oocyte, which grows as its sister cells regress and die. Although critical for fertility, the biological and physical mechanisms underlying this transport process are poorly understood. Here, we combined live imaging of germline cysts, genetic perturbations, and mathematical modeling to investigate the dynamics and mechanisms that enable directional and complete cytoplasmic transport in Drosophila melanogaster egg chambers. We discovered that during "nurse cell (NC) dumping" most cytoplasm is transported into the oocyte independently of changes in myosin-II contractility, with dynamics instead explained by a

SUBMITTER: Imran Alsous J 

PROVIDER: S-EPMC7958293 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature