Anomalous nanoparticle surface diffusion in LCTEM is revealed by deep learning-assisted analysis.

ABSTRACT: The motion of nanoparticles near surfaces is of fundamental importance in physics, biology, and chemistry. Liquid cell transmission electron microscopy (LCTEM) is a promising technique for studying motion of nanoparticles with high spatial resolution. Yet, the lack of understanding of how the electron beam of the microscope affects the particle motion has held back advancement in using LCTEM for in situ single nanoparticle and macromolecule tracking at interfaces. Here, we experimentally studied the motion of a model system of gold nanoparticles dispersed in water and moving adjacent to the silicon nitride membrane of a commercial LC in a broad range of electron beam dose rates. We find that the nanoparticles exhibit anomalous diffusive behavior modulated by the electron beam dose rate. We

SUBMITTER: Jamali V 

PROVIDER: S-EPMC7958372 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature