PDX models reflect the proteome landscape of pediatric acute lymphoblastic leukemia but divert in select pathways.

ABSTRACT:

Background

Murine xenografts of pediatric leukemia accurately recapitulate genomic aberrations. How this translates to the functional capacity of cells remains unclear. Here, we studied global protein abundance, phosphorylation, and protein maturation by proteolytic processing in 11 pediatric B- and T- cell ALL patients and 19 corresponding xenografts.

Methods

Xenograft models were generated for each pediatric patient leukemia. Mass spectrometry-based methods were used to investigate global protein abundance, protein phosphorylation, and limited proteolysis in paired patient and xenografted pediatric acute B- and T- cell lymphocytic leukemia, as well as in pediatric leukemia cell lines. Targeted next-generation sequencing was utilized to examine genetic abnormalities in pati

SUBMITTER: Uzozie AC 

PROVIDER: S-EPMC7958471 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature