Maternal protein restriction induces renal AT2R promoter hypomethylation in salt-sensitive, hypertensive rats.

ABSTRACT:

Scope

We previously demonstrated that protein restriction in utero induced salt-sensitive hypertension and changed renal levels of angiotensin type 2 receptor (AT2R) in Stroke-Prone Spontaneously Hypertensive Rat (SHRSP). Here, we investigated if this characteristic alteration of AT2R is related to AT2R DNA methylation profiles.

Methods and results

First, we examined the relation between AT2R DNA methylation and its promoter activity in vitro. Luciferase assays revealed a negative correlation between these two variables. Next, we fed SHRSP dams and grand-dams a control 20% casein diet or a 9% casein diet during pregnancy. Adult offspring and grand-offspring were supplied either water or 1% saline solution for 2 weeks. Renal AT2R promoter DNA near the TATA-box was hypo

SUBMITTER: Miyoshi M 

PROVIDER: S-EPMC7958563 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature