Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana.

ABSTRACT: Gene silencing in plants using topical dsRNA is a new approach that has the potential to be a sustainable component of the agricultural production systems of the future. However, more research is needed to enable this technology as an economical and efficacious supplement to current crop protection practices. Systemic gene silencing is one key enabling aspect. The objective of this research was to better understand topically-induced, systemic transgene silencing in Nicotiana benthamiana. A previous report details sequencing of the integration site of the Green Fluorescent Protein (GFP) transgene in the well-known N. benthamiana GFP16C event. This investigation revealed an inadvertent co-integration of part of a bacterial transposase in this line. To determine the effect of this transgene c

SUBMITTER: Hendrix B 

PROVIDER: S-EPMC7959375 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature