Electrochemical Genosensing of E. coli Based on Padlock Probes and Rolling Circle Amplification.

ABSTRACT: Isothermal amplification techniques are emerging nowadays for the rapid and accurate detection of pathogenic bacteria in low resource settings, where many infectious diseases are endemic, and the lack of reliable power supply, trained personnel and specialized facilities pose critical barriers for timely diagnosis. This work addresses the detection of E. coli based on DNA isothermal amplification performed on magnetic particles (MPs) followed by electrochemical genosensing on disposable electrodes by square-wave voltammetry. In this approach, the bacterial DNA is preconcentrated using a target-specific magnetic probe and then amplified on the MPs by rolling circle amplification (RCA). Two different electrochemical readout methods for the RCA amplicons are tested. The first one relie

SUBMITTER: Ben Aissa A 

PROVIDER: S-EPMC7959471 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature