Engineering a Virus-like Particle to Display Peptide Insertions Using an Apparent Fitness Landscape.

ABSTRACT: Peptide insertions in the primary sequence of proteins expand functionality by introducing new binding sequences, chemical handles, or membrane disrupting motifs. With these properties, proteins can be engineered as scaffolds for vaccines or targeted drug delivery vehicles. Virus-like particles (VLPs) are promising platforms for these applications since they are genetically simple, mimic viral structure for cell uptake, and can deliver multiple copies of a therapeutic agent to a given cell. Peptide insertions in the coat protein of VLPs can increase VLP uptake in cells by increasing cell binding, but it is difficult to predict how an insertion affects monomer folding and higher order assembly. To this end, we have engineered the MS2 VLP using a high-throughput technique, called Systematic

SUBMITTER: Robinson SA 

PROVIDER: S-EPMC7959485 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature