DOT1L-controlled cell-fate determination and transcription elongation are independent of H3K79 methylation.

ABSTRACT: Actively transcribed genes in mammals are decorated by H3K79 methylation, which is correlated with transcription levels and is catalyzed by the histone methyltransferase DOT1L. DOT1L is required for mammalian development, and the inhibition of its catalytic activity has been extensively studied for cancer therapy; however, the mechanisms underlying DOT1L's functions in normal development and cancer pathogenesis remain elusive. To dissect the relationship between H3K79 methylation, cellular differentiation, and transcription regulation, we systematically examined the role of DOT1L and its catalytic activity in embryonic stem cells (ESCs). DOT1L is dispensable for ESC self-renewal but is required for establishing the proper expression signature of neural progenitor cells, while catalytic ina

SUBMITTER: Cao K 

PROVIDER: S-EPMC7959542 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature