Low Rho activity in hepatocytes prevents apical from basolateral cargo separation during trans-Golgi network to surface transport.

ABSTRACT: Hepatocytes, the main epithelial cells of the liver, organize their polarized membrane domains differently from ductal epithelia. They also differ in their biosynthetic delivery of single-membrane-spanning and glycophosphatidylinositol-anchored proteins to the apical domain. While ductal epithelia target apical proteins to varying degrees from the trans-Golgi network (TGN) to the apical surface directly, hepatocytes target them first to the basolateral domain, from where they undergo basolateral-to-apical transcytosis. How TGN-to-surface transport differs in both scenarios is unknown. Here, we report that the basolateral detour of a hepatocyte apical protein is due, in part, to low RhoA activity at the TGN, which prevents its segregation from basolateral transport carriers. Activating Rho

SUBMITTER: Lazaro-Dieguez F 

PROVIDER: S-EPMC7959587 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature