Deletion of Glutamine Synthetase Gene Disrupts the Survivability and Infectivity of Leishmania donovani.

ABSTRACT: Glutamine synthetase (GS) is one of the most important metabolic enzymes which catalyzes ligation of glutamate and ammonia to form glutamine. Previous studies from our lab had revealed significant differences in parasite and host GS enzyme which warranted us to further work on its relevance in parasite. To analyze glutamine synthetase function in Leishmania, we generated GS overexpressors and knockout mutants and evaluated their ability to grow in vitro in monocyte differentiated macrophage and in vivo by infections in BALB/c mice. GS knocked out strain showed significant growth retardation with delayed cell cycle progression and morphological alteration. Null mutants exhibited attenuated infectivity both in in vitro and in vivo experiments and the effect

SUBMITTER: Kumar V 

PROVIDER: S-EPMC7959746 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature