Determining the quantitative relationship between glycolysis and GAPDH in cancer cells exhibiting the Warburg effect.

ABSTRACT: Previous studies have identified GAPDH as a promising target for treating cancer and modulating immunity because its inhibition reduces glycolysis in cells (cancer cells and immune cells) with the Warburg effect, a modified form of cellular metabolism found in cancer cells. However, the quantitative relationship between GAPDH and the aerobic glycolysis remains unknown. Here, using siRNA-mediated knockdown of GAPDH expression and iodoacetate-dependent inhibition of enzyme activity, we examined the quantitative relationship between GAPDH activity and glycolysis rate. We found that glycolytic rates were unaffected by the reduction of GAPDH activity down to 19% ± 4.8% relative to untreated controls. However, further reduction of GAPDH activity below this level caused proportional reductions in

SUBMITTER: Zhu X 

PROVIDER: S-EPMC7960551 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature