Nicotine exposure increases markers of oxidant stress in stored red blood cells from healthy donor volunteers.

ABSTRACT:

Background

Cigarette smoking is a frequent habit across blood donors (approx. 13% of the donor population), that could compound biologic factors and exacerbate oxidant stress to stored red blood cells (RBCs).

Study design and methods

As part of the REDS-III RBC-Omics (Recipient Epidemiology Donor Evaluation Study III Red Blood Cell-Omics) study, a total of 599 samples were sterilely drawn from RBC units stored under blood bank conditions at Storage Days 10, 23, and 42 days, before testing for hemolysis parameters and metabolomics. Quantitative measurements of nicotine and its metabolites cotinine and cotinine oxide were performed against deuterium-labeled internal standards.

Results

Donors whose blood cotinine levels exceeded 10 ng/mL (14% of the tested donors) were c

SUBMITTER: Stefanoni D 

PROVIDER: S-EPMC7960685 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature