Aurora Kinase B Expression, Its Regulation and Therapeutic Targeting in Human Retinoblastoma.

ABSTRACT:

Purpose

Aurora kinase B (AURKB) plays a pivotal role in the regulation of mitosis and is gaining prominence as a therapeutic target in cancers; however, the role of AURKB in retinoblastoma (RB) has not been studied. The purpose of this study was to determine if AURKB plays a role in RB, how its expression is regulated, and whether it could be specifically targeted.

Methods

The protein expression of AURKB was determined using immunohistochemistry in human RB patient specimens and immunoblotting in cell lines. Pharmacological inhibition and shRNA-mediated knockdown were used to understand the role of AURKB in cell viability, apoptosis, and cell cycle distribution. Cell viability in response to AURKB inhibition was also assessed in enucleated RB specimens. Immunoblotting was em

SUBMITTER: Borah NA 

PROVIDER: S-EPMC7960835 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature