Binding of calcium and magnesium to human cardiac troponin C.

ABSTRACT: Cardiac muscle thin filaments are composed of actin, tropomyosin, and troponin that change conformation in response to Ca²⁺ binding, triggering muscle contraction. Human cardiac troponin C (cTnC) is the Ca²⁺-sensing component of the thin filament. It contains structural sites (III/IV) that bind both Ca²⁺ and Mg²⁺ and a regulatory site (II) that has been thought to bind only Ca²⁺. Binding of Ca²⁺ at this site initiates a series of conformational changes that culminate in force production. However, the mechanisms that underpin the regulation of binding at site II remain unclear. Here, we have quantified the interaction between site II and Ca²⁺/Mg²⁺ through isothermal titration calorimetry and thermodynami

SUBMITTER: Rayani K 

PROVIDER: S-EPMC7961095 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature