Mass spectrometry imaging of phosphatidylcholine metabolism in lungs administered with therapeutic surfactants and isotopic tracers.

ABSTRACT: Mass spectrometry imaging (MSI) visualizes molecular distributions throughout tissues but is blind to dynamic metabolic processes. Here, MSI with high mass resolution together with multiple stable isotope labeling provided spatial analyses of phosphatidylcholine (PC) metabolism in mouse lungs. Dysregulated surfactant metabolism is central to many respiratory diseases. Metabolism and turnover of therapeutic pulmonary surfactants were imaged from distributions of intact and metabolic products of an added tracer, universally ¹³C-labeled dipalmitoyl PC (U¹³C-DPPC). The parenchymal distributions of newly synthesized PC species were also imaged from incorporations of methyl-D₉-choline. This dual labeling strategy demonstrated both lack of inhibition of endogenous

SUBMITTER: Ellis SR 

PROVIDER: S-EPMC7961103 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature