A murine model to study the gut bacteria parameters during complex antibiotics like cefotaxime and ceftriaxone treatment.

ABSTRACT:

Background

The globally increasing resistance due to extended-spectrum beta-lactamase producing Enterobacteriaceae is a major concern. The objective of this work was to develop a murine model to study the gut bacteria parameters during complex antibiotics like cefotaxime and ceftriaxone treatment and to compare the fecal carriage of ESBL-producing Enterobacteriaceae.

Methods

SWISS mice were treated either with ceftriaxone or with cefotaxime or with NaCl 0.9% as a control group from day 1 to day 5. We performed a gavage at day 4 with a Klebsiella pneumonia CTX-M9. We collected stools and performed pharmacological measurements, cultures and 16S rRNA gene amplification and sequencing during the 12 days of the stool collection.

Results

Mice treated wi

SUBMITTER: Gregoire M 

PROVIDER: S-EPMC7961304 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature