Lipid profiles of autophagic structures isolated from wild type and Atg2 mutant Drosophila.

ABSTRACT: Autophagy is mediated by membrane-bound organelles and it is an intrinsic catabolic and recycling process of the cell, which is very important for the health of organisms. The biogenesis of autophagic membranes is still incompletely understood. In vitro studies suggest that Atg2 protein transports lipids presumably from the ER to the expanding autophagic structures. Autophagy research has focused heavily on proteins and very little is known about the lipid composition of autophagic membranes. Here we describe a method for immunopurification of autophagic structures from Drosophila melanogaster (an excellent model to study autophagy in a complete organism) for subsequent lipidomic analysis. Western blots of several organelle markers indicate the high purity of the isolated autophagic vesicl

SUBMITTER: Laczko-Dobos H 

PROVIDER: S-EPMC7961809 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature