CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral integration sites.

ABSTRACT: Lentiviruses have been widely used as a means of transferring exogenous DNAs into human cells to treat various genetic diseases. Lentiviral vectors are fundamentally integrated into the host genome, but their integration sites are generally unpredictable, which may increase the uncertainty for their use in therapeutics. To determine the viral integration sites in the host genome, several PCR-based methods have been developed. However, the sensitivities of the PCR-based methods are highly dependent on the primer sequences, and optimized primer design is required for individual target sites. In order to address this issue, we developed an alternative method for genome-wide mapping of viral insertion sites, named CReVIS-seq (CRISPR-enhanced Viral Integration Site Sequencing). The method is ba

SUBMITTER: Kim HS 

PROVIDER: S-EPMC7961857 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature