Establishment of 3D Neuro-Organoids Derived from Pig Embryonic Stem-Like Cells.

ABSTRACT: Although the human brain would be an ideal model for studying human neuropathology, it is difficult to perform in vitro culture of human brain cells from genetically engineered healthy or diseased brain tissue. Therefore, a suitable model for studying the molecular mechanisms responsible for neurological diseases that can appropriately mimic the human brain is needed. Somatic cell nuclear transfer (SCNT) was performed using an established porcine Yucatan EGFP cell line and whole seeding was performed using SCNT blastocysts. Two Yucatan EGFP porcine embryonic stem-like cell (pESLC) lines were established. These pESLC lines were then used to establish an in vitro neuro-organoids. Aggregates were cultured in vitro until 61 or 102 days after neural induction, neural patterning, and neural expa

SUBMITTER: Hwang SU 

PROVIDER: S-EPMC7961951 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature